The OKUNO Lab

Department of Material and Biological Chemistry Faculty of Yamagata University

【原著論文】

27.Ikeda Y., Hama S.*, Kajimoto K., Okuno T., Tsuchiya H., Kogure K., Quantitative comparison of Adipocytokine gene     expression during adipocyte maturation in non-obes and obese rats. Biol. Pharm. Bull. (in press)

26.Ohgita T., Okuno T.*, Hama S., Tsuchiya H., Kogure K., A new method to evaluate the unfolding activity of  chaperone unit ClpA based on Fe-S cluster disruption. Chem. Pharm. Bull., 59, 657-661 (2011).

25.Sakurai T.*, Iwasaki T., Okuno T., Kawata Y., Kise N., Eavaluation of Aβ fibrillization inhibitory effect by a PEG-peptide conjugated based on an Aβ-peptide fragment with intramolecular FRET. Chem. Commun., 47, 4709-4711(2011).

24.Okuno T.*, Ohgita T., Sasa T., Nonaka A., Funasaki N., Kogure K., Fluorescence polarization analysis for revealing molecular mechanism of nucleotide-dependent phospholipid membrane binding of MinD ATPase. Biol. Pharm. Bull., 33, 1746-1750 (2010).

23.Ayuso-Tejedor S., Nishikori S., Okuno T., Ogura T., Sancho J.*, FtsH cleavage of non-native conformations of proteins. J. Struct. Biol., 171, 117-124 (2010).

22.Okuno T.*, Yamabayashi H., Kogure K., Comparison of intracellular localization between Nubp1 and Nubp2 utilizing GFP fusion protein. Mol.Biol.Rep., 37, 1165-1168 (2010).

21.Okuno T.*, Ogoh M., Tanina H., Funasaki N., Kogure K., Direct monitoring of interaction between Escherichia coli proteins, MinC and monomeric FtsZ, in solution. Biol. Pharm. Bull., 32, 1473-1475 (2009).

20.Okuno T., Yamanaka K., Ogura T.*, Flavodoxin, a new fluorescent substrate for monitoring proteolytic activity of FtsH lacking a robust unfolding activity., J. Struct. Biol., 156, 115-119 (2006).

19.Okuno T., Yamanaka K., Ogura T.*, Characterization of mutants of the Escherichia coli AAA protease, FtsH, carrying a mutation in the central pore region., J. Struct. Biol., 156, 109-114 (2006).

18.Funasaki N.*, Uratsuji I., Okuno T., Hirota S., Neya S., Masking mechanisms of bitter taste of drugs studied with ion selective electrodes., Chem. Pharm. Bull., 54, 1155-1161 (2006).

17.Funasaki N.*, Fukuba M., Hattori T., Ishikawa S., Okuno T., Hirota S., Micelle formation of bile salts and zwitterionic derivative as studied by two-dimensional NMR spectroscopy., Chem. Phys. Lipids., 142, 43-57 (2006).

16.Okuno T., Yamanaka K., Ogura T.*, An AAA protease FtsH can initiate proteolysis from internal sites of a model substrate, apo-flavodoxin., Genes Cells, 11, 261-268 (2006).

15.Sakamoto W., Miura E., Kaji Y., Okuno T., Nishizono M., Ogura T., Allelic characterization of the leaf-variegated mutation var2 identifies the conserved amino acid residues of FtsH that are important for ATP hydrolysis and proteolysis., Plant. Mol. Biol., 56, 705-716 (2005).

14.Okuno T., Yamada-Inagawa T., Karata K., Yamanaka K., Ogura T., Spectrometric analysis of degradation of a physiological substrate 32 by Escherichia coli AAA protease FtsH., J. Struct. Biol., 146, 148-154 (2004).

13.Yamada-Inagawa T., Okuno T., Karata K., Yamanaka K., Ogura T., Conserved pore residues in the AAA protease, FtsH, are important for proteolysis and its coupling to ATP hydrolysis., J. Biol.Chem., 278, 50182-50187 (2003).

12.Okuno T., Hirota H., Yamauchi O., Folding Character of Cytochrome c Studied by o-Nitrobenzyl Modification of Methionine 65 and Subsequent Ultraviolet Light Irradiation., Biochemistry, 39, 7538-7545 (2000).

11.Hirota S., Hayamizu K., Okuno T., Kishi M., Iwasaki H., Kondo T., Hibino T., Takabe T., Kohzuma T., Yamauchi O., Spectroscopic and Electrochemical Studies on Structural Change of Plastocyanin and Its Tyrosine 83 Mutants Induced by Interaction with Lysine Peptides., Biochemistry, 39, 6357-6364 (2000).

10.Okuno T., Hirota H., Yamauchi O., Folding Properties of Cytochrome c Studied by Photocleavable o-Nitrobenzyl Modification of Methionine 65 and 80., Chem. Lett., 290-291 (2000).

9.Kobayashi T., Okuno T., Suzuki T., Kumita M., Ohba S., Nishida Y., DNA Degradation by the Copper (II) Complex with Tripodal-ligands Containing Peptide Group., Polyhedron, 17, 1553-1559 (1998).

8.Nishida Y., Itoh S., Okuno T., Ohba S., New Insight into Reaction of Iron (III)-peroxide Adduct with Alkans. An Alternative Model for Cytochrome P450 and Methane Monooxygenase., Z. Naturforsh., 52,

  612-615 (1997).

7.Okuno T., Itoh S., Ohba S., Nishida Y., μ-Oxo Bridged Diiron(III) Complexes and Hydrogen Peroxide: Oxygenation and Catalase-like Activities., J. Chem. Soc.,Dalton Trans., 3547-3551 (1997).

6.Okuno T., Ohba S., Nishida Y., Oxidation of Cyclohexane with Hydrogen Peroxide Catalysed by Copper(II) Complexes Containing N,N-Bis(2-pyrydylmethyl)-alanineamide Ligands., Polyhedron, 16, 3765-3774 (1997).

5.Itoh S., Okuno T., Itoh H., Matsushima H., Tokii N., Nishida Y., Chemical Basis for High Activity in Oxygenation of Cyclohexane Catalyzed by Dinuclear Iron (III) Complexes with Ethereal Oxygen Containing Ligand and Hydrogen Peroxide System., Z. Naturforsh., 52, 719-727 (1997).

4.Itoh S., Okuno T., Matsushima H., Tokii N., Nishida Y., Chemical Origin of High Activity in Oxygenation of Cyclohexane by H2O2 Catalysed by Dinuclear Iron (III) Complexes with Amidecontaining Ligands., J. Chem. Soc., Dalton Trans., 4479-4484 (1996).

3.Itoh S., Okuno T., Matsushima H., Tokii N., Nishida Y., Isolation of Two Geometrical Isomers of Bis(μ-acetato) (μ-oxo)Diiron(III) Complexes with Tridentate Ligands and Their Activities for Functionalization of Cyclohexane in the Presence of Hydrogen Peroxide., J. Chem. Soc., Dalton Trans., 4037-4039 (1996).

2.Okuno T., Nishida Y., Electron Delocalization in Binuclear Manganese(III/IV) Complexes with di-μ-oxo Bridges., Polyhedron, 15,1509-1515 (1996).

1.Nishida Y., Okuno T., Itoh S., Harada A., Ohba S., Matsushima H., Tokii N., Important Role of Tetrahydrofuran Ring in Activation of Hydrogen Peroxide in the Presence of Binuclear Iron(III) Complexes with Linear μ-oxo Bridge., Chem.Lett., 885-886 (1995).


【総説,著書】

#    Ogura, T., Okuno, T., Suno, R., and Akiyama, Y. FtsH protease. In Handbook of Proteolytic Enzymes, 3rd edition, N. Rawlings and G. Salvesen(ed) Elsevier Ltd., in press.

#   Okuno, T. and Ogura, T. FtsH protease, a eubacterial membrane-bound AAA protease. In ATP-dependent proteases (Research Signport, ed. E. Kutejova) pp.87-114, 2008.

   

【和文総説等】

#     奥野貴士, 山田-稲川知子, 小椋光,「AAATPaseの構造とタンパク質リモデリングの分子機構」,生物物理, Vol. 43, No.5 (2003).


#        奥野貴士, 蛋白質科学会シンポジウム報告「エネルギー変換マシーンの作動原理」, Life of Proteins, No10 (2003)



【国際学会発表 (2011年~)】

#     Ohgita T., Okuno T., Hama S., Tsuchiya H., Kogure K., An improved evaluation system for the unfolding activity of chaperone unit ClpA using Fe-S protein Ferredoxin as a model substrate., The EMBO meeting2011, Vienna, September, 2011


【国内学会•研究会発表 (2011年~)】

#      Okuno T, Sugawara Y, Takahashi S, Kato A, Adachi I, Ueno M, Development of model cell membrane system for in vitro analysis of membrane.,  KEY Forum in Developmental Biology and Regenerative Medicine, 熊本, 2011. 9. 8~9.

#    Ohgita T., Okuno T., Hama S., Tsuchiya H., Kogure K., Ferredoxin をモデル基質とする大腸菌シャペロンClpAの新規アンフォールディング活性評価法の確立, 第49回日本生物物理学会年会,兵庫県立大学姫路書写キャンパス,  2011.9.

#  奥野貴士: 分光学的手法による膜蛋白質機能解析に関する研究, 第17回日本膜学研究奨励賞 受賞講演, 東京, 2011.5.12~13.

#      高橋慎太郎, 奥野貴士, 上野雅晴: 界面活性物質が生体膜機能に及ぼす影響, 日本膜学会第33年会, 東京,

        2011.5.12~13.